General description

The homodimeric S-100 isoform composed of ββ-subunits, purified from bovine brain. The S-100 proteins are acidic Ca²⁺ binding proteins found predominately in glial cells. Evidence indicates that upon binding to Ca²⁺, S-100 proteins expose a hydrophobic domain to the solvent in a manner similar to calmodulin. Within the cell, S-100 proteins have been reported to regulate adenylate cyclase, aldolase activities, ATPase, microtubules, and protein phosphorylation. Under nonreducing conditions both the dimeric and monomeric forms may be visible.r>r>Note: This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.

Native homodimeric S-100b isoform composed of ββ-subunits, purified from bovine brain. Elevated serum level of S-100b is used as a marker of stroke and cerebral edema.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Momotani, E., et al. 1993. J. Comp. Pathol. 108, 291.Baudier, J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 11627.Donato, R. 1991. Cell Calcium12, 713.Isobe, T., et al. 1977. Biochim. Biophys. Acta494, 222.

Packaging

1 mg in Plastic ampoule

Physical form

Lyophilized from 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 100 µM DTT.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Standard Handling (A)